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Suggested strategies for signi cant improvement in liquid blending arising from experimentation in the scaled-down tank included retro tting the small diameter high power number Rushton with a large diameter low power number axial ow impeller such as an up-pumping Chemineer HE3 or a Prochem Max o T, the use of increased energy dissipation rates, and an increased impeller clearance ratio C/T of 05 For caustic pH control, alkali is generally added at the liquid surface at the later stages of cell culture processes, and it is here that pH excursions are known to occur, especially if liquid blending is poor Langheinrich and Nienow (1999) also used the geometrically scaled down 061 m tank to investigate possible pH excursions in the 8 m3 bioreactor At the operating conditions described earlier, they found signi cant pH excursions of up to 0.

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8 pH unit at the point of alkali addition in a buffered system using both pH probe measurements and ow visualization studies Such excursions can be detrimental for cell viability and product formation (Brown and Birch, 1996; Osman et al, 2001, 2002) The pH variations were absent when alkali was added to the impeller region They also found that an increase in the overall energy dissipation rate did not have a signi cant impact on the pH excursions given the substantial differences in the local energy dissipation rates at the liquid surface and the impeller region The authors recommended that the pH excursions could, realistically, be avoided only with alkali added to the impeller region via a pipe Traditionally, this option has not been implemented in industrial scale bioreactors due to clean in place (CIP) considerations..

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109. E. G. Kuntz, Cherntech 17, 570 (1987).

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